De novo engineered guide RNA-directed transposition with TnpB-family proteins reveals features of naturally evolved systems
Richard D. Schargel, Laura Chacon Machado, Shravanika Kumaran, Jordan E. Thesier, Alba Guarné, Joseph E. Peters
This paper engineers de novo RNA-guided transposition systems using TnpB-family proteins, showing improved specificity and efficiency compared to naturally evolved systems.
bioRxiv · Aug 27, 2025 · PreprintMolecular Biology