Modulation of SpyCatcher Ligation Kinetics by SpyTag Thioamide Substitution
Kristen E. Fiore, Denver Y. Francis, Sobrielle M. Casimir, Ryann M. Perez, E. James Petersson
This study investigates how thioamide substitutions in SpyTag affect ligation kinetics with SpyCatcher, showing both faster and slower complex formation depending on substitution position.
bioRxiv · Oct 10, 2024 · PreprintBiochemistry