Detergent-free reconstitution of transmembrane proteins in giant liposomes of complex curvature by the Synthetic Membrane Transfer
Hemraj Meena, Rafal Zdanowicz, Tobias Bock-Bierbaum, Till Stephan, Sofie Dorothea Ottsen Knudsen, Stefan Jakobs, Oliver Daumke, Per Amstrup Pedersen, Nicola De Franceschi
This paper introduces a detergent-free method to reconstitute transmembrane proteins into GUVs with complex curvature, enabling functional studies of membrane proteins in physiologically relevant geometries.
bioRxiv · Aug 23, 2025 · PreprintSynthetic Biology