PermaPhosSer: autonomous synthesis of functional, permanently phosphorylated proteins
Phillip Zhu, Stanislau Stanisheuski, Rachel Franklin, Amber Vogel, Cat Hoang Vesely, Patrick N. Reardon, Nikolai N. Sluchanko, Joseph S. Beckman, P. Andrew Karplus, Ryan A. Mehl, Richard B. Cooley
The paper develops a system to autonomously produce phosphorylated proteins in E. coli, demonstrating that non-hydrolyzable phosphoserine mimics phosphoserine function in three biological contexts.
ACS Central Science · Apr 10, 2023Peer-reviewedSynthetic Biology