A simplified two-plasmid system for orthogonal control of gene expression using light-activated CRISPR effector for mammalian cells
Shruthi Satya Garimella, Shiaki A. Minami, Anusha N Khanchandani, Justin C. Abad Santos, Susannah R. Schaffer, Priya S. Shah
This paper simplifies the LACE optogenetic system from four to two plasmids, showing similar performance with reduced variability.
BMC Biotechnology · Jul 1, 2025Peer-reviewedBioengineering