Mechanistic Modeling Explains the Production Dynamics of Recombinant Adeno-Associated Virus with the Baculovirus Expression Vector System
Francesco Destro, John Joseph, Prasanna Srinivasan, Joshua M. Kanter, Caleb I. Neufeld, Jacqueline M. Wolfrum, Paul W. Barone, Stacy L. Springs, Anthony J. Sinskey, Sylvain Cecchini, Robert M. Kotin, Richard D. Braatz
The paper develops a mechanistic model of rAAV production in BEVS, identifying Rep78 levels and transgene expression as key factors limiting yields.
Molecular Therapy — Methods & Clinical Development · Jun 2, 2023Peer-reviewedMolecular Biology