Author

John Joseph

Brigham and Women's HospitalORCID 0000-0002-0232-6042Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Signature Assays, Organisms & Methods

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Research Focus:drug response / drug discovery· 3 Approach:experimental assay· 3 Research Focus:cell type annotation· 2 Research Focus:clustering· 1 Organism:mouse· 1 Organism:plant (other)· 1 Organism:rat· 1 Approach:statistical model· 1

Frequent Co-Authors

Anthony J. SinskeyiD· 2 shared Caleb I. Neufeld· 2 shared Francesco DestroiD· 2 shared Jacqueline M. WolfrumiD· 2 shared James N. LuoiD· 2 shared Jeffrey M. KarpiD· 2 shared Mickael DangiD· 2 shared Nitin JoshiiD· 2 shared

Activity by Year

2023-2026 · peak 2/yr

Co-Author Collaboration Network

10 top collaborators · 21 mutual co-author links

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Focal author (center)Selected co-authorORCID co-authorUnlinked name

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2 shared papersBioengineering

Anthony J. SinskeyORCID

Mutual co-authors in cluster

Author profile →

4 papers

Recent·Most cited

Continuous Production of Recombinant Adeno-Associated Virus in the Insect Cell/Baculovirus Expression Vector System

John Joseph, Francesco Destro, Arella Yuan, Daniel Antov, Wenyu Chen, Sally Song, Matthew Theriault, Chengcheng Yuan, Alexander Sansom, Chiara Lundin, Tyler Burns, Hadeel Abubaker Hamed, Piero Morales, Jessica Xinyuan Fan, Avner Romero Rodriguez, , Stacy L. Springs

This study develops a continuous production process for recombinant adeno-associated virus (rAAV) using the Sf9/BEVS system, achieving stable yields over 4 weeks.

Molecular Therapy Advances · May 1, 2026Peer-reviewedBioengineering

A Precisely Controlled Long-Acting Immunosuppression Platform Enables Prolonged Survival of Vascularized Composite Allografts

Sohyung Lee, Fatih Zor, Yalçın Külahçı, John Joseph, Hüseyın Karagöz, Shumaim Barooj, Gunjan Malik, Dongsung Park, James N. Luo, Lingyun Zhu, Ally Wang, Krisco Chung, Nancy Li, Purna Shah, Eli Agus, , Vijay S. Gorantla

PRECISE, a long-acting immunosuppression platform, maintains therapeutic drug levels and prolongs graft survival in VCA models through precise control of tacrolimus and rapamycin release.

bioRxiv · Nov 7, 2025 · PreprintBioengineering

A Mechanically Resilient Soft Hydrogel Improves Drug Delivery for Treating Post-Traumatic Osteoarthritis in Physically Active Joints

Nitin Joshi, Jing Yan, Mickael Dang, Kai V. Slaughter, Yufeng Wang, Dana Wu, Trevor Wai-Ming Ung, Bhingaradiya Nutan, Virja Pandya, Mu Xian Chen, Shahdeep Kaur, Sachin H. Bhagchandani, Haya A. Alfassam, John Joseph, Jingjing Gao, , Joerg Ermann

This paper develops a mechanically resilient hydrogel for drug delivery in physically active joints, showing sustained release and reduced cartilage degeneration in a mouse model of post-traumatic osteoarthritis.

Proceedings of the National Academy of Sciences · Mar 31, 2025Peer-reviewedBioengineering

Mechanistic Modeling Explains the Production Dynamics of Recombinant Adeno-Associated Virus with the Baculovirus Expression Vector System

Francesco Destro, John Joseph, Prasanna Srinivasan, Joshua M. Kanter, Caleb I. Neufeld, Jacqueline M. Wolfrum, Paul W. Barone, Stacy L. Springs, Anthony J. Sinskey, Sylvain Cecchini, Robert M. Kotin, Richard D. Braatz

The paper develops a mechanistic model of rAAV production in BEVS, identifying Rep78 levels and transgene expression as key factors limiting yields.

Molecular Therapy — Methods & Clinical Development · Jun 2, 2023Peer-reviewedMolecular Biology