BiochemistrybioRxiv
Label free fluorescence quantification of hydrolytic enzyme activity on native substrates reveal how lipase function depends on membrane curvature
S. S.- R. Bohr, C. Thorlaksen, R. M. Kuhnel…·N. S. Hatzakis
This paper introduces a fluorescent assay to measure lipase activity on native substrates by detecting changes in membrane environment, revealing how membrane curvature and lid region mutations affect lipase function.
DOI 10.1021/acs.langmuir.0c00787