A Real-Time Analysis of Protein Transport via the Twin Arginine Translocation Pathway in Response to Different Components of the Protonmotive Force in vivo
W. Zhou, B. Hao, T. M. Bricker, S. M. Theg
This study develops a real-time assay to analyze the energy requirements of the Tat pathway in E. coli, showing that both ΔpH and Δψ contribute to protein transport in vivo.
bioRxiv · Jan 12, 2023Biochemistry