Simultaneous readout of multiple FRET pairs using photochromism
T. Roebroek, W. Vandenberg, F. Sipieter, S. Hugelier, C. Stove, J. Zhang, P. Dedecker
The paper presents a method to simultaneously measure multiple FRET pairs using donor photochromism, validated with biosensors for PKA, ERK, and calcium.
bioRxiv · Jan 6, 2021Biophysics