W. Vandenberg

Novel Genetically Encoded Probes for Functional Imaging of Cell Signaling by Polarization Microscopy

This paper introduces FLIPs, a new class of genetically encoded biosensors that enable real-time single-cell imaging of cell signaling without modifying target proteins.

Per-pixel unmixing of spectrally overlapping fluorophores using intra-exposure excitation modulation

The paper introduces exNEEMO, a method to quantify spectrally overlapping fluorophores using intra-exposure excitation modulation, enabling accurate per-pixel unmixing.

The TriScan: fast and sensitive 3D confocal fluorescence imaging using a simple optical design

The paper introduces a fast and sensitive 3D confocal fluorescence microscope called TriScan, combining speed and resolution for various applications.

Fast and artifact-free excitation multiplexing using synchronized image scanning

The paper introduces a device called Resonator for fast, artifact-free fluorescence imaging with multiple excitation wavelengths, demonstrating its use in monitoring light-induced dynamics and Ca2+ signaling.

Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting

The paper introduces a method to enhance fluorescence imaging by splitting the PSF into multiple copies, enabling simultaneous multicolor imaging with super-resolution and single-molecule capabilities.

Photochromic fluorophores enable imaging of lowly-expressed proteins in the autofluorescent fungus Candida albicans

The paper uses photochromic fluorophores and SOFI to image lowly-expressed proteins in C. albicans despite high background fluorescence.

Self-contained and modular structured illumination microscope

The paper introduces a modular structured illumination microscope (SIM) called HIT-SIM that enhances existing fluorescence microscopes with fast super-resolution imaging capabilities.

Simultaneous readout of multiple FRET pairs using photochromism

The paper presents a method to simultaneously measure multiple FRET pairs using donor photochromism, validated with biosensors for PKA, ERK, and calcium.

Integrated structure-function dataset reveals key mechanisms underlying photochromic fluorescent proteins

The paper develops 27 photochromic fluorescent proteins with varied spectroscopic properties and determines their crystal structures to analyze structure-function relationships.

A Modular Approach to Active Focus Stabilization for Fluorescence Microscopy

This paper presents a modular, cost-effective system for detecting and correcting z-drift in fluorescence microscopy, demonstrating its effectiveness through temperature perturbation and simulated stage movement tests.

SOFIevaluator: a strategy for the quantitative quality assessment of SOFI data

SOFIevaluator quantitatively assesses SOFI data quality and identifies effective fluorescent proteins for SOFI imaging.

Quantitative comparison of camera technologies for cost-effective Super-resolution Optical Fluctuation Imaging (SOFI)

This study compares camera technologies for cost-effective Super-resolution Optical Fluctuation Imaging (SOFI), showing that industrial-grade detectors can achieve SOFI on standard and home-built microscopes.