S. Hugelier

Super-Resolution Imaging Uncovers Nanoscale Tau Aggregate Hyperphosphorylation Patterns in Human Alzheimers Disease Brain Tissue

The paper uses super-resolution imaging to analyze nanoscale tau aggregates in AD and PART brain tissues, revealing hyperphosphorylation patterns and morphological differences.

Multiplexed DNA-PAINT Imaging of the Heterogeneity of Late Endosome/Lysosome Protein Composition

This study uses multiplexed DNA-PAINT imaging to reveal cell-type specific subpopulations of late endosomes/lysosomes with unique protein compositions.

ECLiPSE: A Versatile Classification Technique for Structural and Morphological Analysis of Super-Resolution Microscopy Data

ECLiPSE is a machine learning pipeline for classifying super-resolution microscopy data, achieving high accuracy in identifying cellular structures and applying to tau protein clearance and TDP-43 morphotypes.

Simultaneous multicolor fluorescence imaging using duplication-based PSF splitting

The paper introduces a method to enhance fluorescence imaging by splitting the PSF into multiple copies, enabling simultaneous multicolor imaging with super-resolution and single-molecule capabilities.

Detyrosination enrichment on microtubule subsets is established by the interplay between a stochastically-acting enzyme and microtubule stability

The paper investigates how detyrosination of microtubules occurs on specific subsets through stochastic enzyme activity and microtubule stability interactions.

Self-contained and modular structured illumination microscope

The paper introduces a modular structured illumination microscope (SIM) called HIT-SIM that enhances existing fluorescence microscopes with fast super-resolution imaging capabilities.

Simultaneous readout of multiple FRET pairs using photochromism

The paper presents a method to simultaneously measure multiple FRET pairs using donor photochromism, validated with biosensors for PKA, ERK, and calcium.

Integrated structure-function dataset reveals key mechanisms underlying photochromic fluorescent proteins

The paper develops 27 photochromic fluorescent proteins with varied spectroscopic properties and determines their crystal structures to analyze structure-function relationships.

Mechanistic investigation of mEos4b reveals a strategy to reduce track interruptions in sptPALM

This paper identifies a long-lived dark state in mEos4b that disrupts sptPALM tracks and shows that 488-nm light can revert it, improving track length and reducing interruptions.