Live Cell Multicolour Lifetime Imaging Using Genetically Encodable Fluorophores
T. Starling, I. Carlon-Andres, M. Iliopoulou, D. J. Williamson, S. Padilla-Parra
The paper demonstrates simultaneous imaging of nine fluorescent proteins using FLIM and PIE, and introduces a method to unmix spectrally similar FPs pixel-by-pixel.
bioRxiv · Oct 6, 2022 · PreprintBiophysics