Live Cell Multicolour Lifetime Imaging Using Genetically Encodable Fluorophores
T. Starling, I. Carlon-Andres, M. Iliopoulou, D. J. Williamson, S. Padilla-Parra
Abstract
Nine fluorescent proteins (FPs) simultaneous imaging has been demonstrated in a single acquisition using fluorescence lifetime imaging microscopy (FLIM) combined with pulsed interleaved excitation (PIE) for three laser lines. We also show how to unmix spectrally similar FPs in a pixel-by-pixel manner with an analytical non-fitting solution.
02 /Specification & Classification
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