Robust Sequence Determinants of α-Synuclein Toxicity in Yeast Implicate Membrane Binding
Robert W. Newberry, Taylor Arhar, Jean Costello, George C. Hartoularos, Alison M. Maxwell, Zun Zar Chi Naing, Maureen Pittman, Nishith R. Reddy, Daniel M. C. Schwarz, Douglas R. Wassarman, Taia S. Wu, Daniel Barrero, Christa Caggiano, Adam Catching, Taylor B. Cavazos, , Jenna Pell
The paper uses deep mutational scanning in yeast to show that a toxic conformation of α-synuclein is stable under chemical perturbations, while mutations affecting toxicity are influenced by these perturbations.
bioRxiv · May 1, 2020Biochemistry