Rapid and scalable preparation of bacterial lysates for cell-free gene expression
Andriy Didovyk, Taishi Tonooka, Lev S. Tsimring, Jeff Hasty
This paper introduces a method for rapidly producing bacterial lysates for cell-free gene expression using autolysis and freeze-thaw or freeze-dry cycles, enabling scalable and cost-effective biosensor development.
ACS Synthetic Biology · Aug 10, 2017Peer-reviewedSynthetic Biology