Jeff Hasty

This paper introduces LRQ, a long-read metagenomics method that enables absolute strain abundance measurement and resolves microbial evolution in a Crohn's disease patient over eight years.

The paper develops a framework using evolved microbial diversity and machine learning to enable combinatoric biosensing of multiple analytes in complex environments.

BudFinder uses a masked auto-encoder to detect yeast cell divisions with minimal training data, reducing manual labeling by over five-fold while maintaining accuracy.

This study improves genetic circuit function in E. coli by evolving strains in complex environments using adaptive laboratory evolution.

This study develops a mathematical model to analyze how environmental glucose fluctuations influence yeast aging and identifies strategies to enhance cellular longevity.

This paper engineers bacteria to detect tumor DNA through horizontal gene transfer, achieving 100% discrimination between mice with and without colorectal cancer.

This study shows that using plasmids with identical origins of replication in synthetic bacterial systems can enable population adaptation through copy number coupling and memory.

This study uses native E. coli as chassis to deliver transgenes, inducing persistent physiological changes in conventionally-raised hosts.

The paper develops a workflow combining directed mutagenesis and microfluidics to create and tune dynamic gene circuits, resulting in a robust genetic oscillator with controlled period-amplitude characteristics.

The paper investigates how CRISPR-Cas systems in Acinetobacter baylyi inhibit natural transformation through altruistic group defense and self-sacrifice, revealing a trade-off between genetic acquisition and immune defense.

The paper shows that interactions between chromatin silencing and mitochondrial pathways create an epigenetic landscape that determines cell fate during aging, and introduces a genetically engineered long-lived state.

The paper presents a rapid, scalable method to construct multiplex natural CRISPR arrays, demonstrating its utility in harnessing the CRISPR system of Acinetobacter baylyi for DNA exclusion and genome editing.

This paper designs gene circuits to control spatiotemporal growth phenotype patterns in microbial colonies, demonstrating oscillations between growth and dormancy and enabling selective gene expression in non-dividing cells.

The paper tracks replicative aging in yeast cells and finds that intermittent heterochromatin silencing loss regulates lifespan, with Sir2 deacetylase playing a key role.

This paper introduces a method for rapidly producing bacterial lysates for cell-free gene expression using autolysis and freeze-thaw or freeze-dry cycles, enabling scalable and cost-effective biosensor development.