Report on pre-validation of an animal-free alternative method (NAM) for regulatory safety testing: InfiniteLungDT, an in-vitro-learned digital twin for the prediction of material-triggered chronic neutrophilic lung inflammation
Iztok Urbančič, Tilen Koklič, Hana Kokot, Boštjan Kokot, Nika Kozoderec, Tomasz Kolodziej, Tomaž Ličina, Lan Ma‐Hock, Pernille Høgh Danielsen, Keld Alstrup Jensen, Mateja Čubej Gašparin, Tereza Pahor, Frédéric Cosnier, Sarah Valentino, Carole Seidel, , Janez Štrancar
The paper pre-validates an animal-free method, InfiniteLungDT, which predicts chronic neutrophilic lung inflammation using in vitro data, achieving high accuracy in LOAEL determination.
bioRxiv · May 17, 2026 · PreprintPharmacology & Toxicology