Compartmentalized CRISPR Reactions (CCR) for High-Throughput Screening of Guide RNA Potency and Specificity
Tinku Supakar, Ashley Herring‐Nicholas, Eric A. Josephs
This paper introduces CCR, a method to screen gRNA potency and specificity in vitro by confining CRISPR reactions in microemulsions, enabling high-throughput validation of large gRNA variant libraries.
Small · Jun 6, 2024Peer-reviewedBioengineering