Author

Ashley Herring‐Nicholas

University of North Carolina at Greensboro

Listed by name: these papers carry no ORCID for this author, so different people with the same name may be mixed together.

Biology Disciplines

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Bioengineering· 2

Signature Assays, Organisms & Methods

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Approach:experimental assay· 2 Research Focus:benchmarking· 1 Approach:genetic perturbation / CRISPR· 1 Research Focus:perturbation response prediction· 1 Research Focus:software / tool· 1

Frequent Co-Authors

Eric A. JosephsiD· 2 shared Stephanie Fisher-Huynh· 1 shared Tinku Supakar· 1 shared

Activity by Year

2024-2025 · peak 1/yr

Co-Author Collaboration Network

3 top collaborators · 2 mutual co-author links

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2 shared papersBioengineering

Eric A. JosephsORCID

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2 papers

Recent·Most cited

BioengineeringbioRxivPreprintMay 6, 2025

TOP-SECRETS enables Cas9 nucleases to discriminate SNVs outside of PAMs

Ashley Herring‐Nicholas, Stephanie Fisher-Huynh, Eric A. Josephs

TOP-SECRETS enables Cas9 to distinguish SNVs outside PAMs by generating gRNA variants that improve discrimination between healthy and disease-associated SNVs.

DOI 10.1101/2025.05.06.652491

experimental assaygenetic perturbation / CRISPR

𝕏 PostBluesky

BioengineeringSmallPeer-reviewedJun 6, 2024

Compartmentalized CRISPR Reactions (CCR) for High-Throughput Screening of Guide RNA Potency and Specificity

Tinku Supakar, Ashley Herring‐Nicholas, Eric A. Josephs

This paper introduces CCR, a method to screen gRNA potency and specificity in vitro by confining CRISPR reactions in microemulsions, enabling high-throughput validation of large gRNA variant libraries.

2 citations

experimental assayperturbation response prediction

𝕏 PostBluesky