Author

Eric A. Josephs

University of North Carolina at GreensboroORCID 0000-0002-5330-6842Unclaimed ORCID profile · follow to get notified of new preprints

Biology Disciplines

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Bioengineering· 2 Plant Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 3 Research Focus:benchmarking· 1 Approach:genetic perturbation / CRISPR· 1 Research Focus:perturbation response prediction· 1 Organism:plant (other)· 1 Research Focus:software / tool· 1

Frequent Co-Authors

Ashley Herring‐Nicholas· 2 shared Ayalew Ligaba‐OsenaiD· 1 shared Stephanie Fisher-Huynh· 1 shared Tasmia Islam· 1 shared Tinku Supakar· 1 shared

Activity by Year

2024-2025 · peak 2/yr

Co-Author Collaboration Network

5 top collaborators · 3 mutual co-author links

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2 shared papersBioengineering

Ashley Herring‐Nicholas

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3 papers

Recent·Most cited

Systematic Optimization Enables Near-Perfect In Vitro Transformation Efficiencies for Spirodela polyrhiza (Greater Duckweed)

Tasmia Islam, Ayalew Ligaba‐Osena, Eric A. Josephs

This study optimizes in vitro transformation protocols for Spirodela polyrhiza, achieving high transformation efficiencies and rapid regeneration of genetically modified plants.

bioRxiv · Sep 10, 2025 · PreprintPlant Biology

TOP-SECRETS enables Cas9 nucleases to discriminate SNVs outside of PAMs

Ashley Herring‐Nicholas, Stephanie Fisher-Huynh, Eric A. Josephs

TOP-SECRETS enables Cas9 to distinguish SNVs outside PAMs by generating gRNA variants that improve discrimination between healthy and disease-associated SNVs.

bioRxiv · May 6, 2025 · PreprintBioengineering

Compartmentalized CRISPR Reactions (CCR) for High-Throughput Screening of Guide RNA Potency and Specificity

Tinku Supakar, Ashley Herring‐Nicholas, Eric A. Josephs

This paper introduces CCR, a method to screen gRNA potency and specificity in vitro by confining CRISPR reactions in microemulsions, enabling high-throughput validation of large gRNA variant libraries.

Small · Jun 6, 2024Peer-reviewedBioengineering