Applying the auxin-inducible degradation (AID) system for rapid protein depletion in mammalian cells
Bramwell G. Lambrus, Tyler C. Moyer, Andrew J. Holland
This paper describes a method to rapidly and reversibly deplete proteins in mammalian cells using the AID system, with a protocol for CRISPR/Cas9-mediated insertion of the AID degron.
bioRxiv · Aug 31, 2017 · PreprintCell Biology