Rapid and efficient isolation of intact engineered extracellular vesicles encapsulating functional proteins via internal twin Strep tetraspanin tagging and Strep technology
M Pham, Jona Benjamin Krohn, Janis Meyer, Christian Ritter, Martin Schneider, Dominic Helm, Dario L. Frey, Uta Haselmann-Weiss, Florian Leuschner, Karl Rohr, Ralf F. W. Bartenschlager
The paper develops a method to isolate intact engineered extracellular vesicles with high purity using twin-Strep tagging and Strep technology, enabling efficient and selective capture and functional delivery of proteins.
bioRxiv · Nov 26, 2025 · PreprintMolecular Biology