Fundamental limits of amplification-free CRISPR-Cas12 and Cas13 diagnostics
D. A. Huyke, A. Ramachandran, V. I. Bashkirov, E. K. Kotseroglou, T. Kotseroglou, J. G. Santiago
This study measures kinetic parameters and limits of detection for CRISPR-Cas12 and Cas13 enzymes in amplification-free diagnostics, revealing typical catalytic efficiencies and picomolar detection limits.
bioRxiv · Jan 31, 2022 · PreprintBioengineering