Heterologous expression and purification of glutamate decarboxylase-1 from the model plant Arabidopsis thaliana - characterization of the enzyme's in vitro truncation by thiol endopeptidase activity
B. S. Menard, K. H. Bendickson, L. M. Raytek, W. A. Snedden, W. C. Plaxton
This paper investigates proteolytic truncation of recombinant AtGAD1 during purification and its impact on enzyme activity.
bioRxiv · Apr 11, 2024 · PreprintBiochemistry