CRISPR-Cas9 mediated nuclear transport and genomic integration of nanostructured genes in human primary cells
E. Lin Shiao, W. G. Pfeifer, B. R. Shy, M. S. Doost, E. Chen, V. S. Vykunta, J. R. Hamilton, E. C. Stahl, D. M. Lopez, C. R. S. Espinoza, A. E. Dejanov, R. J. Lew, M. G. Poirer, A. Marson, C. E. Castro, J. A. Doudna
This study delivers DNA nanostructures into human primary cells using CRISPR-Cas9, achieving efficient genomic integration with lower toxicity than dsDNA.
bioRxiv · Nov 8, 2021 · PreprintBioengineering