Complete Reconstitution and Deorphanization of the 3 MDa NOCAP (NOCardiosis-Associated Polyketide) Synthase
K. P. Yuet, C. W. Liu, S. R. Lynch, J. Kuo, W. Michaels, R. B. Lee, A. E. McShane, B. L. Zhong, C. R. Fischer, C. Khosla
The paper reconstitutes and deorphanizes a nonamodular PKS from Nocardia strains, identifying the NOCAP aglycone structure through in vitro and heterologous expression methods.
bioRxiv · Jan 28, 2020Biochemistry