Fast and ultra-sensitive glycoform analysis by supercritical fluid chromatography-tandem mass spectrometry
Y. Haga, M. Yamada, R. Fujii, N. Saichi, T. Yokokawa, T. Hama, Y. Hayakawa, K. Ueda
This study develops a fast, sensitive SFC-MS/MS method to analyze glycoforms of therapeutic mAbs, detecting 102 glycoforms with a 5 attomole limit and a 6-order dynamic range.
bioRxiv · Sep 30, 2021 · PreprintBiochemistry