Burden-driven feedback control of gene expression
Francesca Ceroni, Alice Boo, Simone Furini, Thomas E. Gorochowski, Olivier Borkowski, Yaseen Ladak, Ali R. Awan, Charlie Gilbert, Guy-Bart V. Stan, Tom Ellis
The paper identifies heat-shock promoters that activate rapidly in response to synthetic gene expression in E. coli and uses them to build a CRISPR/dCas9-based feedback system that improves protein yield by adjusting synthetic construct expression based on cellular burden.
Nature Methods · Mar 26, 2018Peer-reviewedSynthetic Biology