Charlie Gilbert

This paper develops a scalable transposon mutagenesis system for non-model bacteria, identifying functional configurations and applying them to study gene function in Comamonas testosteroni.

This paper develops a genetic toolkit for Sporosarcina pasteurii, enabling genome modification and uncovering genes involved in biocementation.

This paper develops an automated platform using active learning to discover electroporation protocols for non-model microbes, achieving higher transformation efficiency for industrial chassis.

This study develops genetic tools for Piscinibacter sakaiensis to enable functional genomics and engineering for PET degradation.

The paper identifies functional plasmids for Vibrio natriegens using the POSSUM toolkit, showing compatibility of multiple replicons in wild-type and engineered strains.

The paper designs and constructs a pan-microbial genetic toolkit using high-throughput methods to identify functional genetic parts in non-model microbes.

The paper introduces a scalable framework to identify functional origins of replication in non-model bacteria using a combinatorial plasmid library and next-generation sequencing.

This paper develops a synthetic symbiotic co-culture system to engineer bacterial cellulose with programmable functional properties.

The paper identifies heat-shock promoters that activate rapidly in response to synthetic gene expression in E. coli and uses them to build a CRISPR/dCas9-based feedback system that improves protein yield by adjusting synthetic construct expression based on cellular burden.

This paper demonstrates a method for tunable extracellular self-assembly of multi-protein conjugates using Bacillus subtilis, enhancing enzyme resilience and enabling functional multi-protein complex formation.