A cloning-free method for CRISPR/Cas9-mediated genome editing in fission yeast
Xiaoran Zhang, Jia-Bei He, Yi-Zheng Wang, Li‐Lin Du
This paper introduces a cloning-free CRISPR/Cas9 method for genome editing in fission yeast using gap repair to assemble plasmids and sgRNA inserts.
G3 Genes Genomes Genetics · Apr 27, 2018Peer-reviewedGenetics