In situ Membrane Protein Expression by Efficient Recruitment of mRNA to the Membranes of Synthetic Cells
Hang Fu, Lijuan Ma, Chunhua Xu, Jinghua Li, Yunhai Sun, Yuanxiao Tao, Hao Wang, Shuxin Hu, Meifang Fu, Hai Zheng, Chenli Liu, Fangfu Ye, Yan Qiao, Ming Li, Ying Hui Lu
This paper introduces a method to enhance membrane protein synthesis in synthetic cells by recruiting mRNA to phospholipid membranes using cholesterol-modified DNA.
bioRxiv · Nov 23, 2024 · PreprintSynthetic Biology