Hai Zheng

DNA replication initiation causally sets the added mass for cell division in Escherichia coli

This paper shows that DNA replication initiation in E. coli sets a fixed mass increment for cell division, resolving conflicting models of division timing.

Extrusion-modulated DnaA activity oscillations coordinate DNA replication with biomass growth

This study links DnaA activity oscillations to DNA replication initiation in E. coli, showing they persist independently of dnaA expression and proposing an extrusion model for regulation.

In situ Membrane Protein Expression by Efficient Recruitment of mRNA to the Membranes of Synthetic Cells

This paper introduces a method to enhance membrane protein synthesis in synthetic cells by recruiting mRNA to phospholipid membranes using cholesterol-modified DNA.

Stabilization of Integrator/INTAC by the small but versatile DSS1 protein

This study identifies DSS1 as an essential subunit of the INTAC complex, showing its role in stabilizing INTAC and its impact on transcriptional regulation.

Catalytic-independent functions of INTAC in conferring sensitivity to BET inhibition

The paper identifies that BET inhibitor efficacy depends on the INTAC complex's auxiliary module, which interacts with the RACK7/ZMYND8-KDM5C complex to remove H3K4 methylation, thereby restoring sensitivity to BET inhibitors through WDR5 degradation.

Structural basis of INTAC-regulated transcription

The paper determines the structure of the PEC-INTAC complex and shows how INTAC facilitates RNA cleavage and CTD dephosphorylation during transcription termination.