Collagen assembly and turnover imaged with a CRISPR-Cas9 engineered Dendra2 tag
Adam Pickard, Antony Adamson, Yinhui Lu, Joan Chang, Richa Garva, Nigel Hodson, Karl E. Kadler
The paper uses CRISPR-Cas9 to tag collagen-I with Dendra2 for live imaging, showing cells synthesize, migrate on, and interact with collagen fibrils.
bioRxiv · Jun 5, 2018 · PreprintCell Biology