Enhancing the Identification of NHS Ester-Mediated Lysine-Cysteine Cross-Linking via Reduced Trypsin Digestion Time
Xin-Feng Yang, Ao-Cheng Hou, Qian Sun, Peng-Zhi Mao, Yujiao Qin, Xiaoming Zhang, Guang-Can Shao
This study shows NHS ester cross-linkers can reliably form lysine-cysteine cross-links in XL-MS, improving protein structure analysis by capturing both K-K and K-C distance constraints.
bioRxiv · Sep 3, 2026 · PreprintBiochemistry