Limit-pushing overexpression reveals constraints on protein abundance
Shotaro Namba, Yuki Nishida, Charles M. Boone, Brenda Andrews, Hisao Moriya
The paper uses a limit-pushing approach in yeast to measure protein abundance and toxicity, finding that overexpression tolerance varies with protein characteristics and can lead to distinct intracellular states.
bioRxiv · Sep 28, 2026 · PreprintSystems Biology