Shotaro Namba

Limit-pushing overexpression reveals constraints on protein abundance

The paper uses a limit-pushing approach in yeast to measure protein abundance and toxicity, finding that overexpression tolerance varies with protein characteristics and can lead to distinct intracellular states.

Amino acid homorepeats act as buffers to maintain proteostasis and constrain the compatible sequence space of proteomes

The paper evaluates the neutrality of amino acid homorepeats in yeast, finding hydrophobic repeats are harmful while hydrophilic repeats suppress proteotoxic stress and define compatible sequence space.

Impact of Maximal Overexpression of a Non-toxic Protein on Yeast Cell Physiology

This study identifies non-toxic proteins in yeast that can be overexpressed without growth impairment, revealing metabolic and ribosomal changes under protein burden.

Toxicity of the model protein 3xGFP arises from degradation overload, not from aggregate formation

This study shows that 3xGFP overproduction causes cytotoxicity through degradation overload, not aggregate formation, as it traps Hsp70 and disrupts the ubiquitin-proteasome system.

Improving the Z3EV promoter system to create the strongest yeast promoter

The paper improves the Z3EV promoter system to create the strongest yeast promoter, P36, which is 1.4 times stronger than TDH3 and can express up to 50% of total protein as a heterologous protein.

Genetic Profiling of Protein Burden and Nuclear Export Overload

The paper identifies genetic interactions associated with protein overproduction and nuclear export overload in yeast using genetic profiling.