Author

Zacharias S. Nielsen

Novo Nordisk Foundation

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Synthetic Biology· 1

Signature Assays, Organisms & Methods

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Approach:experimental assay· 1 Research Focus:variant calling· 1 Assay:whole-genome sequencing· 1

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Ekaterina KozaevaiD· 1 shared Manuel Nieto‐DomíngueziD· 1 shared Pablo I. NikeliD· 1 shared

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Ekaterina KozaevaORCID

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Recent·Most cited

Synthetic BiologyNucleic Acids ResearchPeer-reviewedJan 2, 2024

The pAblo·pCasso self-curing vector toolset for unconstrained cytidine and adenine base-editing in Pseudomonas species

Ekaterina Kozaeva, Zacharias S. Nielsen, Manuel Nieto‐Domínguez, Pablo I. Nikel

The paper develops a CRISPR-based base-editing toolkit for precise single-nucleotide modifications in Gram-negative bacteria, enabling gene inactivation and reversion with plasmid-free strain engineering.

32 citationstop 4%

whole-genome sequencingexperimental assay

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