Manuel Nieto‐Domínguez

This paper demonstrates the use of engineered Pseudomonas putida minicells to convert organic acids into short-chain methyl ketones through pathway optimization and whole-cell biocatalysis.

The paper develops a CRISPR-based base-editing toolkit for precise single-nucleotide modifications in Gram-negative bacteria, enabling gene inactivation and reversion with plasmid-free strain engineering.

This study develops an enzymatic method to produce fluorinated alanine enantiomers with high yield and enantiomeric excess.