Oriented Display of Cello-Oligosaccharides for Pull-down Binding Assays to Distinguish Binding Preferences of Glycan Binding Proteins
Markus Hackl, Zachary Power, Shishir P. S. Chundawat
The paper develops a pull-down binding assay to assess the directional binding preferences of cellulose-binding modules to cello-oligosaccharides, revealing similar affinity for cellohexaose but not cellopentaose.
bioRxiv · Jan 26, 2023Molecular Biology