Shishir P. S. Chundawat

The paper uses N-GLYcanyzer to monitor Trastuzumab biosimilar N-glycoform changes in a microperfusion process, identifying key parameters affecting glycoform evolution.

This study investigates how mechanical pulling affects the unbinding of a cellulosomal carbohydrate-binding module from cellulose using simulations and experiments, showing that pulling orientation significantly impacts unfolding and unbinding dynamics.

The paper demonstrates that chemical rescue is a predictive proxy for glycosynthase activity, validated through correlation with enzymatic activity and transition state analysis.

This study shows that modifying the charge of PET-binding modules can enhance the thermostability and functional persistence of PET hydrolases.

This study improves cellulase activity through protein supercharging, identifying a variant with 2-3-fold higher activity on cellulosic substrates and demonstrating its synergistic potential with endocellulases.

This study determines the reaction kinetics of procainamide labeling of N-glycans to improve analytical workflows for glycoprotein manufacturing.

The paper identifies CSC and REM as mechanisms mediating plant cell resilience through cell wall-plasma membrane attachments under hyperosmotic stress.

This paper visualizes cellulose biosynthesis and microfibril network assembly in live Arabidopsis protoplasts using time-lapse imaging, revealing dynamics of fibril synthesis and network formation.

This paper expresses and characterizes family-2 glycosyltransferases using a cell-free system, showing their catalytic activity and kinetic properties.

The paper develops a pull-down binding assay to assess the directional binding preferences of cellulose-binding modules to cello-oligosaccharides, revealing similar affinity for cellohexaose but not cellopentaose.

The paper introduces a synthetic promoter-based azide biosensor toolkit for in vivo detection of azide anions in E. coli, offering tunable GFP expression and low-cost protein expression.