Synthetic BiologyMethodsPeer-reviewed
Characterizing and Prototyping Genetic Networks with Cell-Free Transcription-Translation Reactions
Melissa K Takahashi, Clarmyra A. Hayes, James Chappell…·Julius B. Lucks
This paper demonstrates the use of cell-free TX-TL systems to rapidly characterize genetic networks, including an RNA transcriptional cascade and a protein-regulated incoherent feed-forward loop.
DOI 10.1016/j.ymeth.2015.05.020