Vincent Noireaux

This paper introduces ORB-TXTL, a cell-free platform for expressing membrane proteins on lipid bilayer-coated beads, demonstrating its utility for studying protein integration and reconstituting metabolic pathways.

This paper engineers red blood cells into synthetic cells using cell-free gene expression to execute gene circuits and synthesize phages.

This paper develops a cell-free platform linking metabolic activity to protein synthesis under resource scarcity, demonstrating how metabolic control can influence gene expression in synthetic cells.

This study creates a synthetic cell-free system where T7 phages infect synthetic cells, demonstrating a complete viral life cycle in vitro.

This paper develops a cell-free biochip platform to profile antibody-antigen interactions and epitope specificity using SARS-CoV-2 antigens and human sera.

This study transplants E. coli chromosomes into artificial cells and shows transcription-dependent swelling and condensin-mediated compaction.

This paper engineers a carbon-conserving cell-free system in E. coli to produce malate from formate and bicarbonate, achieving a 15-fold increase in titer and reducing background reactions by 6-fold.

The paper demonstrates that silicon rhodamines can photoconvert to oxygen rhodamines under red light, causing crosstalk in single-molecule microscopy and introduces methods to reduce this effect.

This paper introduces minireporters for monitoring gene expression and peptide production in cell-free systems, reducing DNA length and synthesis costs while maintaining reliable output.

The paper introduces PHEIGES, a method to rapidly engineer and select phages using cell-free transcription-translation, producing high titers of engineered phages within one day.

The paper demonstrates a single DNA molecule-based genetic circuit that functions as a dissipative nanodevice by enabling coexpressional localization of proteins.

This study shows that decision-making in synthetic cells can shift from slow and precise to rapid and probabilistic by reducing the number of computing molecules to single-molecule levels.

The paper designs and validates an in vitro synthetic gene circuit that uses integral feedback to maintain gene expression at a target level despite disturbances.

The paper introduces DRAFTS, a method for multiplexed transcriptional measurement in cell-free systems, and shows functional differences in gene expression across bacterial species and hybrid lysates.

The paper characterizes two RNA regulators with distinct timescales and demonstrates their use in creating a genetic pulse generator through computational modeling and experimental validation.

The paper shows that gene expression in cell-free reactions scales anomalously with droplet size due to surface effects.

This paper introduces an E. coli cell-free system to rapidly and scalably characterize CRISPR technologies, including DNA cleavage, gene repression, and anti-CRISPR activity.

This study investigates how altering transcription and translation rates in E. coli affects protein expression, revealing that increased initiation rates can reduce expression and that codon bias helps regulate synthesis.

This paper demonstrates the use of cell-free TX-TL systems to rapidly characterize genetic networks, including an RNA transcriptional cascade and a protein-regulated incoherent feed-forward loop.

This study uses cell-free TX-TL systems to rapidly prototype and characterize RNA genetic circuits, showing response times of ~5 minutes per cascade step and demonstrating temporal gene expression staging.