A Cysteine-Less and Ultra-Fast Split Intein Rationally Engineered from Being Aggregation-Prone to Highly Efficient in Protein trans-Splicing
Christoph Humberg, Zahide Yilmaz, Katharina Fitzian, Wolfgang Dörner, Daniel Kümmel, Henning D. Mootz
The paper identifies aggregation as the cause of low splicing efficiency in a cysteine-less split intein and presents a rationally engineered variant with high activity.
Nature Communications · Mar 19, 2025Peer-reviewedBiochemistry