Ana María Restrepo Sierra

This paper engineers synthetic cells capable of DNA self-replication, transcription-translation, and membrane synthesis, showing genetic factors dominate over metabolic interactions in module integration.

The paper demonstrates sustainable replication and Darwinian evolution of a DNA self-replicator in liposome compartments using a cell-free protein synthesis system.

This paper introduces CADGE, a method to enhance gene expression in cell-free systems by amplifying DNA templates, improving protein output and DNA recovery.