Christophe Danelon

The paper optimizes synthetic protein systems in vitro by screening combinatorial DNA variants to identify optimal gene expression and functionality.

The paper describes an image-based workflow to sort synthetic cells based on phenotypes linked to genetic content, using automated microscopy and neural networks.

This study optimizes the PURE system composition using automation and active learning, achieving up to 3-fold improvements in protein yield and translation rate.

The paper designs and builds a partial synthetic genome that can be expressed in a cell-free system and replicated, demonstrating a proof-of-concept for a minimal synthetic cell.

The paper demonstrates that synthetic cells with minimal metabolism can undergo Darwinian evolution by filtering functional genotypes and mapping mutations in a key enzyme.

This paper introduces autocatalytic selection (ACS) to integrate biological functions in vitro by linking gene activity to DNA replication through feedback loops, demonstrating selection of transcription, nucleotide synthesis, and beta-galactosidase activity.

The paper introduces MOSAIC, a method for efficient plasmid editing and library generation using SSAP CspRecT, achieving high editing efficiency and combinatorial library creation in a single step.

This paper engineers synthetic cells capable of DNA self-replication, transcription-translation, and membrane synthesis, showing genetic factors dominate over metabolic interactions in module integration.

The paper demonstrates sustainable replication and Darwinian evolution of a DNA self-replicator in liposome compartments using a cell-free protein synthesis system.

This paper introduces CADGE, a method to enhance gene expression in cell-free systems by amplifying DNA templates, improving protein output and DNA recovery.

The paper shows that MinDE can drive FtsZ-FtsA filament patterns on model membranes without MinC, suggesting a simpler mechanism for Z-ring positioning than previously thought.

This paper demonstrates that bacterial microtubules can be expressed in a cell-free system and used to deform lipid vesicles, offering a new tool for synthetic cell biology.

This paper optimizes the cDICE method to produce high-quality, defect-free GUVs with efficient encapsulation of complex biochemical systems.

This study demonstrates the reconstitution of FtsZ-based division proto-rings in cell-free systems that constrict liposomes, providing insights into bacterial division mechanisms and artificial cell development.

This study demonstrates DNA-programmed membrane synthesis in liposomes using cell-free expressed phospholipid-producing enzymes.