Molecular BiologyBIO-PROTOCOLPeer-reviewed
A one-enzyme RT-qPCR assay for SARS-CoV-2, and procedures for reagent production
Sanchita Bhadra, Andre C. Maranhao, Inyup Paik, Andrew D. Ellington
The paper develops a RT-qPCR assay using a thermostable reverse transcriptase/DNA polymerase (RTX) and provides protocols for reagent production, aiming to improve testing scalability and reliability in resource-limited settings.
24 citations