Cell BiologybioRxivPreprint
Comparative benchmarking of CRISPRi and CasRx in standardized pluripotent stem cell platforms reveals context-dependent knockdown performance
Lu Ni, Takuya Murakami, Satoshi Suzuki…·Kazutoshi Takahashi
This study compares CRISPRi and CasRx for gene knockdown in human pluripotent stem cells, finding CRISPRi provides faster and stronger repression but slower recovery, while CasRx offers reversible knockdown with isoform resolution but lower efficiency.
DOI 10.64898/2026.05.13.724469