Jacques Boutet de Monvel

This paper describes a protocol for in utero AAV-mediated gene delivery to the mouse inner ear, targeting sensory and supporting cells, and standardizes imaging procedures for reproducibility.

This study shows that otoferlin's free C-terminal end is essential for synaptic vesicle docking and fusion in inner hair cells, as demonstrated by deafness and reduced exocytosis in a knock-in mouse model.

The paper introduces Zellige, a software tool for extracting multiple surfaces from 3D microscopy images, demonstrating its performance on synthetic and biological samples.