Ruud A. Weusthuis

This paper introduces a rapid HPLC-based method to measure NAD(P)(H) and NMN redox cofactor concentrations and ratios in microbes, validated against a classical assay and applied to engineered E. coli and Pseudomonas putida.

The paper introduces Rhodo-Box, a toolkit for R. sphaeroides that provides standardized genetic parts and cloning workflows to enhance metabolic engineering.

This study enhances malonyl-CoA availability in Pseudomonas putida through multi-level optimization, achieving a 5.8-fold increase in phloroglucinol production.

This study redesigns bacterial catabolism by repurposing the shikimate pathway for aromatic compound production, achieving over 89% of theoretical yield.